Icosahedral capsid of the phicrAss001 virion
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 7qof
Q-score: 0.575
Bayfield OW, Shkoporov AN, Yutin N, Khokhlova EV, Smith JLR, Hawkins DEDP, Koonin EV, Hill C, Antson AA
Nature (2023) 617 pp. 409-416 [ Pubmed: 37138077 DOI: doi:10.1038/s41586-023-06019-2 ]
- Head fiber dimer protein gp29 (10 kDa, Protein from Bacteroides phage crAss001)
- Major capsid protein gp32 (57 kDa, Protein from Bacteroides phage crAss001)
- Bacteroides phage crass001 (Virus from Bacteroides phage crAss001)
- Head fiber trimer protein gp21 (13 kDa, Protein from Bacteroides phage crAss001)
- Magnesium ion (24 Da, Ligand)
- Auxiliary capsid protein gp36 (36 kDa, Protein from Bacteroides phage crAss001)
Portal protein assembly of the phicrAss001 virion with C12 symmetry imposed
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 7qog
Q-score: 0.583
Bayfield OW, Shkoporov AN, Yutin N, Khokhlova EV, Smith JLR, Hawkins DEDP, Koonin EV, Hill C, Antson AA
Nature (2023) 617 pp. 409-416 [ Pubmed: 37138077 DOI: doi:10.1038/s41586-023-06019-2 ]
- Bacteroides phage crass001 (Virus from Bacteroides phage crAss001)
- Ring protein 2 gp40 (26 kDa, Protein from Bacteroides phage crAss001)
- Portal protein gp20 (93 kDa, Protein from Bacteroides phage crAss001)
- Cargo protein 1 gp45 (90 kDa, Protein from Bacteroides phage crAss001)
- Ring protein 1 gp43 (27 kDa, Protein from Bacteroides phage crAss001)
Unique vertex of the phicrAss001 virion with C5 symmetry imposed
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 7qoh
Q-score: 0.559
Bayfield OW, Shkoporov AN, Yutin N, Khokhlova EV, Smith JLR, Hawkins DEDP, Koonin EV, Hill C, Antson AA
Nature (2023) 617 pp. 409-416 [ Pubmed: 37138077 DOI: doi:10.1038/s41586-023-06019-2 ]
- Portal vertex capsid protein gp57 (11 kDa, Protein from Bacteroides phage crAss001)
- Head fiber trimer protein gp21 (10 kDa, Protein from Bacteroides phage crAss001)
- Major capsid protein gp32 (57 kDa, Protein from Bacteroides phage crAss001)
- Bacteroides phage crass001 (Virus from Bacteroides phage crAss001)
- Magnesium ion (24 Da, Ligand)
- Portal protein gp20 (93 kDa, Protein from Bacteroides phage crAss001)
- Auxiliary capsid protein gp36 (36 kDa, Protein from Bacteroides phage crAss001)
Unique vertex of the phicrAss001 virion
Resolution: 3.62 Å
EM Method: Single-particle
Fitted PDBs: 7qoi
Q-score: 0.505
Bayfield OW, Shkoporov AN, Yutin N, Khokhlova EV, Smith JLR, Hawkins DEDP, Koonin EV, Hill C, Antson AA
Nature (2023) 617 pp. 409-416 [ Pubmed: 37138077 DOI: doi:10.1038/s41586-023-06019-2 ]
- Portal vertex capsid protein gp57 (11 kDa, Protein from Bacteroides phage crAss001)
- Head fiber trimer protein gp21 (10 kDa, Protein from Bacteroides phage crAss001)
- Major capsid protein gp32 (57 kDa, Protein from Bacteroides phage crAss001)
- Cargo protein 1 gp45 (90 kDa, Protein from Bacteroides phage crAss001)
- Bacteroides phage crass001 (Virus from Bacteroides phage crAss001)
- Ring protein 2 gp40 (26 kDa, Protein from Bacteroides phage crAss001)
- Magnesium ion (24 Da, Ligand)
- Portal protein gp20 (93 kDa, Protein from Bacteroides phage crAss001)
- Auxiliary capsid protein gp36 (36 kDa, Protein from Bacteroides phage crAss001)
- Ring protein 1 gp43 (27 kDa, Protein from Bacteroides phage crAss001)
Tail barrel assembly of the phicrAss001 virion with C12 symmetry imposed
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 7qoj
Q-score: 0.562
Bayfield OW, Shkoporov AN, Yutin N, Khokhlova EV, Smith JLR, Hawkins DEDP, Koonin EV, Hill C, Antson AA
Nature (2023) 617 pp. 409-416 [ Pubmed: 37138077 DOI: doi:10.1038/s41586-023-06019-2 ]
- Tail hub protein b gp39 (13 kDa, Protein from Bacteroides phage crAss001)
- Bacteroides phage crass001 (Virus from Bacteroides phage crAss001)
- Ring protein 2 gp40 (26 kDa, Protein from Bacteroides phage crAss001)
- Tail fiber protein gp22 (92 kDa, Protein from Bacteroides phage crAss001)
- Magnesium ion (24 Da, Ligand)
- Ring protein 4/5 gp34 (26 kDa, Protein from Bacteroides phage crAss001)
- Portal protein gp20 (93 kDa, Protein from Bacteroides phage crAss001)
- Water (18 Da, Ligand)
- Ring protein 3 gp35 (26 kDa, Protein from Bacteroides phage crAss001)
- Cargo protein 1 gp45 (90 kDa, Protein from Bacteroides phage crAss001)
- Ring protein 1 gp43 (27 kDa, Protein from Bacteroides phage crAss001)
- Tail hub protein a gp38 (24 kDa, Protein from Bacteroides phage crAss001)
Tail muzzle assembly of the phicrAss001 virion with C6 symmetry imposed
Resolution: 3.38 Å
EM Method: Single-particle
Fitted PDBs: 7qok
Q-score: 0.529
Bayfield OW, Shkoporov AN, Yutin N, Khokhlova EV, Smith JLR, Hawkins DEDP, Koonin EV, Hill C, Antson AA
Nature (2023) 617 pp. 409-416 [ Pubmed: 37138077 DOI: doi:10.1038/s41586-023-06019-2 ]
- Bacteroides phage crass001 (Virus from Bacteroides phage crAss001)
- Magnesium ion (24 Da, Ligand)
- Ring protein 4/5 gp34 (26 kDa, Protein from Bacteroides phage crAss001)
- Muzzle protein gp44 (156 kDa, Protein from Bacteroides phage crAss001)
- Muzzle bound helix (1 kDa, Protein from Bacteroides phage crAss001)
Tail assembly of the phicrAss001 virion with C6 symmetry imposed
Resolution: 3.33 Å
EM Method: Single-particle
Fitted PDBs: 7qol
Q-score: 0.525
Bayfield OW, Shkoporov AN, Yutin N, Khokhlova EV, Smith JLR, Hawkins DEDP, Koonin EV, Hill C, Antson AA
Nature (2023) 617 pp. 409-416 [ Pubmed: 37138077 DOI: doi:10.1038/s41586-023-06019-2 ]
- Tail hub protein b gp39 (13 kDa, Protein from Bacteroides phage crAss001)
- Bacteroides phage crass001 (Virus from Bacteroides phage crAss001)
- Ring protein 2 gp40 (26 kDa, Protein from Bacteroides phage crAss001)
- Tail fiber protein gp22 (92 kDa, Protein from Bacteroides phage crAss001)
- Magnesium ion (24 Da, Ligand)
- Ring protein 4/5 gp34 (26 kDa, Protein from Bacteroides phage crAss001)
- Portal protein gp20 (93 kDa, Protein from Bacteroides phage crAss001)
- Water (18 Da, Ligand)
- Ring protein 3 gp35 (26 kDa, Protein from Bacteroides phage crAss001)
- Muzzle bound helix (1 kDa, Protein from Bacteroides phage crAss001)
- Cargo protein 1 gp45 (90 kDa, Protein from Bacteroides phage crAss001)
- Ring protein 1 gp43 (27 kDa, Protein from Bacteroides phage crAss001)
- Muzzle protein gp44 (156 kDa, Protein from Bacteroides phage crAss001)
- Tail hub protein a gp38 (24 kDa, Protein from Bacteroides phage crAss001)
Asymmetric reconstruction of the phicrAss001 virion
Bayfield OW, Shkoporov AN, Yutin N, Khokhlova EV, Smith JLR, Hawkins DEDP, Koonin EV, Hill C, Antson AA
Nature (2023) 617 pp. 409-416 [ Pubmed: 37138077 DOI: doi:10.1038/s41586-023-06019-2 ]